FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951705
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGACTCCC38190.4012798083439721No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35920.3774278794374307No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA35210.36996758449309397No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30920.3248905910970311No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTGTT29780.31291208935542003No Hit
GTGCAGCCACAGTTCGATTAATCTCCAGACGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTGCTACGCTG28650.3010386621904897No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATC25990.2730888247933971No Hit
GTGCTAACACTGTGAGTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGGAGA25140.2641574857755292No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24970.2623712179719556No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24960.2622661433952748No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24310.25543629591102285No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23770.24976226877025967No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC23100.24272227213264613No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCACAGTGTTAGCAC22630.2377837670286486No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21910.230218397507631No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21740.22843212970405746No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21620.22717123478388787No Hit
GCCTAGAGCCTGAAGATCTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC20240.21267094320193755No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19040.20006199400024166No Hit
CTCCTGCAGGGCCACTCACAGTGTTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGACTCCCAGGCT17790.18692767191514176No Hit
GCGTAGCAACTGGCCTCCGATCACCTTCGGCCAAGGGACACGTCTGGAGATTAATCGAACTGTGGCTGCACCAT17600.1849312549582066No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17600.1849312549582066No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17440.1832500617313138No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16880.17736588543718906No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16200.1702208142228947No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCA16120.1693802176094483No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCGTCCCA16070.1688548447260443No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15820.16622798030902433No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15800.16601783115566274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15560.16349604131532355No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATCTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG15420.16202499724179237No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15170.1593981328247724No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCACAGTGTTAGCACCTACTTAGCCTGGTA15110.1587676853646876No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGATCACCTTCGGCCA14640.15382918026069003No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTAATCTCCAGACGTGTCC14410.15141246499703165No Hit
CGTCTGGAGATTAATCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT14280.15004649550018126No Hit
CTGCTACTCTGGCTCCCAGATGCCACCGGAGAAACTGTGTTGACACAGTC14230.14952112261677727No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14100.14815515311992686No Hit
GCCTGGGAGTCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGGTGCTA14060.14773485481320367No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14040.14752470565984208No Hit
GTAATAAACTGCAAGATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13980.14689425819975727No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13570.1425862005558445No Hit
CTCCAGACGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTGCTACGCTGCTGACAGTAATAAACTGCAAGAT13310.13985426156214373No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13310.13985426156214373No Hit
GGCCGAAGGTGATCGGAGGCCAGTTGCTACGCTGCTGACAGTAATAAACTGCAAGATCTTCAGGCTCTAGGCTG13260.13932888867873974No Hit
CCTTCGGCCAAGGGACACGTCTGGAGATTAATCGAACTGTGGCTGCACCA13050.13712232256844295No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC13010.13670202426171976No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12930.13586142764827336No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12840.13491575645814619No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12740.1338650106913382No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12570.1320787428877646No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12550.131868593734403No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12340.1296620276241062No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12070.12682501405372465No Hit
CTCACAGTGTTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGATG11770.12367277675330066No Hit
TCTATGATGCATCCAACAGGGCCACTGGCGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT11570.12157128521968469No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11330.11904949537934549No Hit
GTAATACACGGCCGTGTCCGCAGTGGTCACAGACCTCAACTTCAGGGAGA11170.1173683021524527No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11100.1166327801156871No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11040.11600233265560231No Hit
CATCATAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTCTGTTGGTACCAG11020.11579218350224073No Hit
GTTGCTACGCTGCTGACAGTAATAAACTGCAAGATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT10960.1151617360421559No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10940.11495158688879432No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGG10930.11484651231211353No Hit
GGCTAAGTAGGTGCTAACACTGTGAGTGGCCCTGCAGGAGAGGGTGGCTC10700.11242979704845514No Hit
TCATAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTCTGTTGGTACCAGGC10590.11127397670496636No Hit
CCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCCTGGGAGTCTGGCC10500.11032830551483916No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10380.10906741059466957No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10370.10896233601798877No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC10320.10843696313458477No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG10300.10822681398122318No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA10290.10812173940454237No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10280.10801666482786157No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10130.10644054617764957No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9620.10108174276692883No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCACCGGAGAAACTGTGTTGACACAGTCTC9560.10045129530684403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2700.044.3999182
GTGGTAT2600.043.4751741
GGTATCA6200.038.1204571
GTATCAA13850.035.61341
CGCTATT851.2848795E-732.24335516
ACGATTA901.9689287E-730.71191840
ATTACTA450.00467174630.60000834
TTCGTTT902.0853258E-730.5113928
GACCGGA1552.5419758E-630.50396770
CTATTAA902.1173037E-730.45846218
CGTTTGT953.31911E-728.93447530
TCCTACG1103.3127435E-828.51985450
TATCAAC17200.028.4764272
CAACGCA17350.028.4276495
AACGCAG17400.028.1505896
ATCAACG17750.027.7855633
AACGATT1004.965659E-727.6319439
TCAACGC17750.027.5926074
CTTCCAT3203.6379788E-1227.43995570
GCTATTA1005.3263284E-727.41117717
Produced by FastQC (version 0.11.5)