FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636824
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCC200.00722445651.98932341
TCTTAGA200.00786857350.87838723
GTAACTT200.00787590750.866415
GTACGAT200.007880799550.858411
AACGTTC305.4535165E-447.38041355
GTATCAA20650.038.913461
ATACAGA350.001468644838.743186
ATGTGTA451.10993984E-437.6669811
GGTATCA8550.037.276341
TATCAAC23200.035.069262
AAAGGTA553.1033455E-431.62036943
GTGGTAT3550.031.5178871
AACGCAG26500.030.9579936
GCCTTAG553.6045755E-430.823281
CTAAAGG450.00451794830.80848741
AGAGTAC26400.029.79115711
CAGAGTA26550.029.75053210
CAACGCA27950.029.3519445
GCAGAGT28000.029.1784559
TTCCATA606.005018E-428.2502372
Produced by FastQC (version 0.11.5)