FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636824
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13580.21324573194477595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTTA100.00854965136.1889370
CGTCTAG200.0077907351.006527
CTATATC200.00787841750.862315
GATATAC200.00788086450.8583034
TAAGGTG200.00788086450.8583035
GTATCAA23800.039.3252031
GTGGTAT4050.038.516041
TATCAAC25350.036.781152
GGTATCA9600.036.7367671
TGGTATC4250.035.899982
GTATAAG502.0586113E-433.9108621
AACGCAG28400.033.1892246
AGAGTAC28350.032.41058311
CAGAGTA28550.032.30483610
GCAGAGT29350.031.8864159
TAAGAGA652.6125153E-531.2974174
CAACGCA30200.031.0987835
TATCGTT450.00469642930.56547536
ACGCAGA30750.030.4346757
CGCAGAG31150.030.043868
Produced by FastQC (version 0.11.5)