FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387876
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.040.5734561
GTATCAA29000.039.421051
CTCGTAT1550.033.51167339
TATCAAC36050.032.091562
TCGTATG1650.031.49550240
CAACGCA37800.030.6969285
AACGCAG38350.030.346476
TCAACGC38550.030.189034
ATCAACG38650.030.0218353
TAGTTAG1253.476624E-530.01603770
AGAGTAC38300.029.84023711
CAGAGTA38600.029.69749610
ACGCAGA40900.028.4513747
TATACAC2300.028.443473
CGCAGAG41200.028.3277688
CTACGTA900.007475250427.79262570
GCAGAGT42450.027.4906449
CGTATGC2150.025.79545841
GTGGTAT4850.024.1393361
AGTACGG40600.023.99600813
Produced by FastQC (version 0.11.5)