FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387876
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17130.12342601212212041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33850.039.45321
GTGGTAT6950.035.6579931
TGGTATC7450.033.2552532
TATCAAC40400.033.217522
GGTATCA14500.032.0460851
GTGTTAG1208.0035534E-1131.5515461
ATCAACG43200.030.8244693
CAACGCA43550.030.7369735
AACGCAG43800.030.7186646
TCAACGC43900.030.4113394
AGAGTAC44250.029.47308311
CAGAGTA45650.028.71996110
CGCAGAG48000.028.030788
ACGCAGA48350.027.7566957
ACGTAAG500.00782373327.5358941
GCAGAGT49250.027.249479
GAGTACG48350.024.55399912
TTAACGG1551.5043042E-924.51446535
AGTACGG48300.024.2944413
TACGGGG20850.021.62045715
Produced by FastQC (version 0.11.5)