FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900902
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53040.5887432817331963No Hit
GAGTTACACTACCCCTGGCACGTTCGGCCAAGGGACCAACGTGGAAATCATACGAACTGTGGCTGCACCAT50570.5613263151818955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40470.44921645195592863No Hit
GTGTAACTCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC38970.4325664722689039No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36140.4011535105927171No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33660.3736255441768361No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30570.339326586021565No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30130.3344425919800378No Hit
GTGCAGCCACAGTTCGTATGATTTCCACGTTGGTCCCTTGGCCGAACGTG29220.32434160430324277No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29150.3235646052511816No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28850.3202346093137766No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28600.3174596126992725No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26790.29736863721026263No Hit
ACACTACCCCTGGCACGTTCGGCCAAGGGACCAACGTGGAAATCATACGA24570.2727266672734659No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23810.26429067756537333No Hit
CTCCTGATCCATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCCAGGTT23760.2637356782424725No Hit
GGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT23690.2629586791904114No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23610.26207068027377006No Hit
AGTCAGAGCATTGCCAGGTTTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATG23480.2606276820342279No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23040.25574368799270064No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAA22990.2551886886697998No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22000.24419970207636346No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21410.23765071006613372No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21070.2338767146704081No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20980.2328777158891866No Hit
TGCCAGGGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAATCTTCA20620.2288817207643007No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19980.2217777294311701No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19810.2198907317333073No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19600.2175597345771238No Hit
ACCCCTGGCACGTTCGGCCAAGGGACCAACGTGGAAATCATACGAACTGTGGCTGCACCATCTGTCTTCATCTTC19350.21478473796261968No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18820.20890174513987092No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17800.19757975895269408No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATTTCCACGTTGGTCC17600.19535976166109079No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17090.18969876856750234No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16660.18492577439055524No Hit
GTCTGCATCTGCAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA16650.18481477452597506No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16520.18337177628643292No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16190.17970878075528748No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACACTACCCCTGGCACGTT16000.17759978332826434No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACACTACC15730.17460278698459988No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15330.17016279240139326No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15290.16971879294307263No Hit
CCCCTGGCACGTTCGGCCAAGGGACCAACGTGGAAATCATACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15230.16905279375559162No Hit
GCAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAGGTTTTTAAATTGGTATCAGCAGAAA15210.16883079402643128No Hit
GTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT14960.16605579741192716No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14620.16228180201620154No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14590.16194880242246104No Hit
GTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14570.1617268026933007No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCGCCATCCTCC14500.16094980364123956No Hit
ACTTATTACTGTCAACAGAGTTACACTACCCCTGGCACGTTCGGCCAAGGGACCAACGTGGAAATCATACGAA14040.15584380987055196No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13950.1548448110893305No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTGG13920.15451181149558996No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13910.15440081163100983No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13840.15362381257894867No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13760.15273581366230732No Hit
GTGGATGCAGCATGGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAAACCTGGCAATG13420.14896181826658172No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTGGATGGGACCCCA13370.1484068189436809No Hit
GATCCATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA13230.14685282083955858No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12730.14130282761055032No Hit
GGACCCCACTTTGCAAAGTGGATGCAGCATGGATCAGGAGCTTAGGGGCT12690.14085882815222964No Hit
GTCCCAGATCCACTGCCACTGAACCTGGATGGGACCCCACTTTGCAAAGT12670.14063682842306932No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT12570.13952682977726769No Hit
GTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAGAGTTACACTACCCCTGGCACGTTCGGCCAAGGGA12510.13886083058978668No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12230.13575283438154206No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCGCCATCCTCCCTGTCTGCATCTGCAGGAGACAGAGTCACCATCA12180.13519783505864122No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12120.13453183587116024No Hit
CTGCATCCACTTTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGA11900.1320898388503966No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11860.13164583939207594No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT11820.13120183993375528No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11710.12998084142337346No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11600.12875984291299164No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11570.12842684331925114No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11550.12820484359009082No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA11380.12631784589222803No Hit
GTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCAC11350.1259848462984875No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11220.12454184805894537No Hit
GGATGGGACCCCACTTTGCAAAGTGGATGCAGCATGGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATA11200.12431984832978503No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11140.12365384914230404No Hit
GCCACAGTTCGTATGATTTCCACGTTGGTCCCTTGGCCGAACGTGCCAGG10960.12165585157986109No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10950.12154485171528091No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10910.12110085225696025No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAG10840.12032385320489909No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10830.12021285334031892No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10790.11976885388199827No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10450.11599485848627264No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10260.11388586105924951No Hit
CCCCTAAGCTCCTGATCCATGCTGCATCCACTTTGCAAAGTGGGGTCCCA10250.11377486119466934No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10200.11321986187176852No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10150.11266486254886769No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10110.11222086309054702No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10060.1116658637676462No Hit
GTCGCCATCCTCCCTGTCTGCATCTGCAGGAGACAGAGTCACCATCACTT10050.11155486390306604No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10030.11133286417390571No Hit
TCCATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT10020.11122186430932554No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10020.11122186430932554No Hit
GCATGGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT10000.1109998645801652No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAAACCTGGCA9780.10855786755940158No Hit
GCCAAGGGACCAACGTGGAAATCATACGAACTGTGGCTGCACCATCTGTC9730.10800286823650077No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCCAGGTT9690.10755886877818008No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9390.10422887284077514No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC9280.10300787433039332No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9250.10267487473665281No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9150.10156487609085116No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9130.10134287636169084No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9120.10123187649711067No Hit
GCTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTGCAGATGCAGACAGGGAGGATGGCGACTGGGTCAT9110.10112087663253051No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAGGTTTTTAAATTGGTATCAGCAGAAACCAGGGAAAG9100.10100987676795034No Hit
TCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAG9090.10089887690337017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1350.043.172591
GTATCAA14850.041.7873731
CTTATAC1400.039.1818471
CGTATGC1150.039.15814641
TATACAC1600.036.422833
TATCAAC17300.035.6673742
TGTTAGA9250.033.32874370
ATCAACG19100.032.306053
TTATACA1700.032.263822
GTCGTAT553.2189835E-431.42559239
TCAACGC19850.031.085424
CAACGCA19900.031.0073155
AACGCAG19950.030.9296046
TCTAAGA450.004768873630.4713862
TAGGGTG450.004768873630.4713865
GCTCAAA850.005486272729.60776970
CGAGACA2000.029.1463523
TGTCGTA605.394209E-428.77457838
CTAGGCC605.627748E-428.5669253
AGAGTAC21300.027.99741611
Produced by FastQC (version 0.11.5)