FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055932
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81150.39471149824021423No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTTCGAAGATT59140.28765542829237545No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACATTA55490.2699019228262413No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54660.2658648243229834No Hit
GTCCCAGACCCACTGCCACTGAATTTGGCTGGGATACCAGTGGCCCTGGG53260.25905526058254846No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51860.25224569684211345No Hit
CTGGTATCCCAGCCAAATTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT51450.25025146746098603No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAAGTC48850.2376051348001782No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48480.23580546438306324No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47750.23225476328983644No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46490.22612615592344493No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTTCGAAGATTCTGCAGTTTATTACTGTCATCAATATGATAACTGG45170.2197057101110348No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45110.21941387166501616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42240.20545426599712444No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC41300.20088213034283234No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40060.19485080245844708No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAATAACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT39880.19397528712039114No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT39130.19032730654515811No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35950.17485986890617006No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34560.16809894490673816No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33310.16201897728134976No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAATTTGGCTGGGATACCA31840.15486893535389304No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAATAACTTAGCCTGGTA31440.15292334571376873No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31370.152582867526747No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30410.1479134523904487No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29770.14480050896624985No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29620.14407091285120324No Hit
GGATACCAGTGGCCCTGGGGGAGGCACCAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGG29040.14124980787302302No Hit
ATATGATAACTGGCCTCAGACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28300.1376504670387931No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC28110.1367263119597341No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27580.1341484056865694No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27530.13390520698155386No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27460.13356472879453213No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27390.13322425060751036No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26010.12651196634908157No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25720.12510141385999146No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25540.12422589852193555No Hit
GTTTATTACTGTCATCAATATGATAACTGGCCTCAGACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA25460.12383678059391069No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25420.12364222162989827No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25310.12310718447886408No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA25290.12300990499685786No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24860.12091839613372427No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24370.11853504882457203No Hit
GTAATAAACTGCAGAATCTTCGAACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA24230.11785409245052851No Hit
CTCCCAGGCTCCTCATCTTTGGTGCCTCCCCCAGGGCCACTGGTATCCCA22900.11138500689711528No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22540.10963397622100342No Hit
GTTATCATATTGATGACAGTAATAAACTGCAGAATCTTCGAACTGCAGGCTGCTGATGGTGAGAGTGAACT22200.10798022502689777No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22150.10773702632188224No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21830.1061805546097828No Hit
CTGCTACTCTGGCTCCCAGACATTACTGGAGAAATAGTGATGACGCAGTC21430.10423496496965853No Hit
CCCCAGGGCCACTGGTATCCCAGCCAAATTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG21050.10238665481154047No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20980.10204617662451872No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20610.10024650620740375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37100.040.4534571
GTGGTAT6450.038.3372421
GGTATCA15800.035.8653371
TATCAAC43600.034.3370972
TCAACGC46400.032.1902474
ATCAACG46450.032.1555983
AACGCAG46500.032.047186
CAACGCA46550.031.9389955
ACCTATA450.00463467130.65157934
AGAGTAC48450.030.40448811
CGATTGT1303.4933102E-529.99667570
CGCAGAG50100.029.6772988
GCAGAGT52000.028.5929349
ACGCAGA53000.028.1830227
GTAGTTT3850.027.00999370
CAGAGTA55200.026.6243410
TATACTG1608.367351E-1125.75225
TTCGGGG14400.025.72631570
TGTTAGA13400.025.70610870
AGTACGG47200.025.53520213
Produced by FastQC (version 0.11.5)