FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547298
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12380.2262021787033755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAG252.5750932E-455.1604542
TAATTCA200.006858880652.67556864
CTATTAC200.00736986551.7271081
GTATCAA15750.043.5711941
TATCAAC17450.039.5132182
GGTATCA5950.038.2519761
TGGTATC2650.036.4267122
GTGCAAT1003.8198777E-1034.4847371
AGAGTAC20000.033.44713611
GTGGTAT2700.033.2075231
CAGAGTA20150.033.1981510
AACGCAG21200.032.3671466
GCAGAGT21250.032.1287239
CAACGCA21850.031.7170035
ATCAACG22500.030.797923
ACGCAGA22600.030.667257
TCTATCA450.004636824630.6446952
CGCAGAG22850.029.7281068
TCAACGC23550.029.7175484
TATAAGA500.00775852127.5802272
Produced by FastQC (version 0.11.5)