FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433404
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC408.2428414E-751.7596230
CCTAAGA408.249408E-751.7536429
TTATACA352.289808E-549.2152522
CTTATAC352.289808E-549.2152521
TAAGACA451.8504124E-646.0298331
ATACACA451.8771025E-645.934234
GTGGTAT1450.045.142261
TATACAC551.4835314E-743.8463133
TCGTATG404.8566886E-543.33333240
GGTATCA5300.042.900841
GTATCAA13350.042.321431
CCCTAAG503.8414746E-641.3933528
GACCCTA503.865951E-641.35512526
TGGTATC1650.039.670472
ATAACGC350.00135408939.38583425
ACCCTAA557.4153504E-637.61293427
TATCAAC15100.036.9603272
CTCGTAT501.8149827E-434.64253639
GATAACG400.002611346534.4546524
TACACAT601.3538231E-534.4506765
Produced by FastQC (version 0.11.5)