FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433404
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7290.16820333914776975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAAT100.009522321131.3585770
TAACTTG252.3415443E-456.22937862
GTTGTAA200.00699167952.41888856
GGTCATA200.007398272451.6749761
ATAACTT305.788142E-446.8082361
GTATCAA14000.044.2928351
TATCAAC15250.040.657582
CCAATAT350.001224928840.19696463
CTATACC350.001357299539.3668674
ACGGGTA901.2369128E-1038.27334216
GGTATCA5200.037.762481
GTGGTAT1900.036.263141
TGGTATC1850.035.376982
GATAACT400.002397223635.06082560
AGAGTAC17750.034.5430411
CAGAGTA17750.034.5430410
GTTTCTA400.002613082334.449981
GTATAAG400.002613082334.449981
GCTATAC400.002614561234.446013
ATAAGAG400.002614561234.446013
Produced by FastQC (version 0.11.5)