FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649490
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC551.4947545E-743.8100281
GTATCAA15550.038.2960431
GGTATCA7100.037.81591
TTATACA754.096728E-836.7169762
GTGGTAT3100.036.6429521
TATACAC807.2283E-834.4221653
TGGTATC3200.034.4221652
TATCAAC17500.033.832072
GTATAGG450.004672565530.597481
GGGTTAG450.004672565530.597487
ATCAACG20500.028.8810353
CAACGCA20400.028.8538745
CTCTTAT605.4917374E-428.6851391
GTCTTAC605.4917374E-428.6851391
AACGCAG20700.028.4357016
TCAACGC21150.027.9934394
CAGAGTA20850.027.73584610
ATAGTAC500.007818175527.5377313
CTCTAAC500.007818175527.5377311
AGAGTAC20950.027.4391511
Produced by FastQC (version 0.11.5)