FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619065
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18460.11401642305898774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.036.636711
TCGTATG2350.033.89336440
CGATTGT1003.4406548E-431.07069670
TATCAAC32900.030.1600652
TCAACGC33900.028.9654874
CGTATGC2650.028.7692341
GTATCAA35550.028.5946731
AACGCAG35100.028.1715286
CAACGCA35150.028.1314565
AATCTCG2250.027.65263236
ATCAACG36800.026.9637533
AGAGTAC37500.026.17994911
TATGCCG2800.026.01463143
ACGCAGA39200.025.2203457
TATACAC3850.025.0572223
CTTATAC3750.024.8104821
CAGAGTA40300.024.61742410
CGCAGAG40650.024.2359858
CTCGTAT2500.023.53317639
AGTACGG36300.023.061913
Produced by FastQC (version 0.11.5)