FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619065
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22010.13594265826263924No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18710.11556052412966744No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT16230.10024304150852499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.034.3075751
TATCAAC35250.029.8758772
TCAACGC34900.029.482474
CAACGCA35350.029.1089635
GTATCAA37850.028.6517121
AACGCAG36500.028.2878636
ATCAACG37550.027.494313
ACGCAGA39900.026.0498857
AGAGTAC39650.025.78012511
CGCAGAG41300.025.000178
CAGAGTA41400.024.85665110
TGGTATC8350.024.7299442
CGATTGT1551.4066392E-424.44452770
GCAGAGT44700.023.252589
GTGGTAT9200.022.8269521
GTGTATA2351.8189894E-1221.9750211
AGTACGG38650.020.83585413
GAGTACG39550.020.62339812
CTATATG4750.019.561483
AATAGCG1451.0754333E-519.39661262
Produced by FastQC (version 0.11.5)