FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063969
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16500.15507970626963755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGTA150.001819077173.64490568
AATAATA150.002203656470.1691258
ATTGTAA200.004014567560.3131669
TAATACA200.006827244552.74067360
ATAATAC200.0068545452.6874959
GAATAAT200.006914614752.57136557
TTTAATG306.424605E-445.834113
GTGGTAT4800.042.9957431
TGGTATC4900.039.991682
GTATCAA29350.039.728951
GGTATCA12450.037.5737881
TATCAAC32600.035.85522
CTCTTTA400.00263292334.40144724
CTTTAAT400.00264143534.378812
TAGACAT450.003897618531.7543867
CAGAGTA35950.030.88833610
AGAGTAC35950.030.69707711
GTGTATA450.00469091130.574751
GTATAAG450.00470495530.5560723
AACGCAG38450.030.2211696
Produced by FastQC (version 0.11.5)