FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999740
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTAA200.00707470652.2677348
CTTATAC1850.050.1452751
CTCTTAT352.3284558E-549.0841
TTATACA1950.047.568962
TATACAC2050.045.2553143
GATTACG405.127095E-542.94422
GTATCAA29300.041.16021
TGTAAAA602.4983274E-741.03996759
TACACAT2500.038.4837765
TCTTATA451.0265555E-438.172622
ATACACA2450.037.866694
TATCAAC32150.037.7214362
GGTATCA11800.037.270561
GTGGTAT4500.031.3046821
TTGTAAA450.00422520831.23495158
AGACTCC3000.030.93375225
AACGCAG39700.030.7253976
TACACAG450.00471491830.5426795
CAGAGTA38450.030.46403110
AGAGTAC38500.030.33524711
Produced by FastQC (version 0.11.5)