FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471479
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC105310.4261011321560895No Hit
GTATAGGAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT93440.37807321041368347No Hit
GTCTATTACTGTGCGAGACTTAGTCGGGGTGGCTCGGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC57350.23204728828365526No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55450.2243595838766989No Hit
GTAATAGACAGTCGTGTCTGTGGCGGTCACAGACGTCATCTTCAGGGAGA53340.21582218582476323No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46110.18656844747618734No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACTCTCACCATCAGCAGC40750.16488102872814214No Hit
GTCTCTGGTGTCTCCATCAGCAATACTGATTACTTCTGGGGCTGGATCCG40410.16350533425531838No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35660.14428607323792758No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35050.14181791550727316No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34630.1401185282173144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34570.13987575860446314No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC34000.13756944728237624No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32970.1334019022617631No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32500.13150020696109496No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32170.13016497409041308No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32110.1299222044775618No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31610.12789912437046805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30890.12498588901625303No Hit
CTCTAAACTAGACGCCTCATACATGATGAGGGTAGGGGGTTTCCCTGGTT30820.1247026578012599No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC29870.12085880559778173No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG29510.11940218792067421No Hit
GGGTAGGGGGTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCA29060.11758141582428983No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA28190.11406125643794666No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27010.10928678738520538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26960.109084479374496No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAA26280.10633309042884848No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26040.10536201197744346No Hit
CCCCCTACCCTCATCATGTATGAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAAT24740.10010200369899966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)