FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376993
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10580.280641815630529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA350.003668677561.67983270
ATAACTC358.767253E-443.02170664
GTATCAA14700.034.0541571
GGTATCA6800.033.8638571
TAACTCG450.002938601733.6324865
ATATCGA502.2612656E-433.368656
GTCTATA450.003290103332.86608560
TATCAAC15500.032.0727042
AACGCAG17100.029.661026
AGAGTAC16850.029.5030811
CAGAGTA16900.029.4157910
GCAGAGT17500.028.979219
GTGGTAT3000.028.9233321
CAACGCA17850.028.2240755
TGGTATC3050.027.3477232
ATCAACG18350.027.0913833
ACGCAGA18950.026.7618037
CGCAGAG19300.026.1036138
TCTTGTA650.001051185425.6647852
TCAACGC19950.025.085884
Produced by FastQC (version 0.11.5)