FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025250
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14280.13928310168251645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAC200.006842138252.71148761
AGAATAC200.007074145652.2688851
AGGTTAG200.00722875951.98387540
TGGTATC5200.041.026282
GTATAAG1350.040.7991071
TATAAGA1350.040.7811972
GGTATCA13500.039.2691421
GTGGTAT5350.039.2500761
GTATCAA31400.039.0287631
ATAAGAG1450.037.966853
TAAGAGA1500.036.7012864
TATCAAC33900.035.728662
TTAGCAC400.002358744635.18129362
AGAGTAC38000.031.42250611
CAGAGTA38000.031.42250610
TGTGATA450.004682477630.58592
GTGATAC450.004683594730.5844083
AACGCAG40050.030.2437136
GCAGAGT40900.029.6993089
CAACGCA41550.029.150465
Produced by FastQC (version 0.11.5)