FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744658
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51150.6868925063586236No Hit
CTCCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC41590.558511424036269No Hit
GTATAGGAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT38440.5162101259907232No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23260.3123581563617124No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACTCTCACCATCAGCAGC16920.22721840093035994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16190.2174152429705986No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14600.19606315919522788No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14530.1951231303497713No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14060.18881150810170574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13660.18343991469909676No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13660.18343991469909676No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13380.1796797993172705No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13380.1796797993172705No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13240.17779974162635734No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13050.17524823476011808No Hit
CTCTAAACTAGACGCCTCATACATGATGAGGGTAGGGGGTTTCCCTGGTT12630.16960806168737863No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12130.1628935699341174No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12110.16262499026398694No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12070.16208783092372606No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11720.1573876866964432No Hit
GGGTAGGGGGTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCA11580.15550762900553006No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10260.13778137077692043No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAA10110.13576702325094203No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10020.13455841473535504No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9290.12475525677559363No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9220.12381522793013706No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9070.12180088040415868No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8690.11669786667168015No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8170.10971479524828848No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAGGAGTTAC8090.1086404765677667No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8070.10837189689763622No Hit
TGTATGAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACT7920.10635754937165787No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7860.10555181036126651No Hit
CCCCCTACCCTCATCATGTATGAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAAT7820.10501465102100561No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7760.10420891201061426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT200.00643049153.54327852
TTGGCGA200.00787030750.8763334
GTTGGCG200.00795117750.7449433
ACGAGCC200.00813213350.4568930
TACTTCG200.00833603250.1417667
TACGGAT2300.046.5802570
GTGGTAT2850.041.0627671
TGATCGG654.535941E-738.03223449
GATCGGA753.1848685E-837.78260450
GTATCAA22150.036.2295151
CGGTTCA609.9939625E-636.0157755
ATCGGAA707.716244E-735.53751851
ATGATCG708.303996E-735.2057448
AATCGCG400.002579649734.5436541
TATCAAC23200.034.4341282
TTAGTCG1850.034.35898620
CGGTTGA1900.033.98702635
CTCGGTT1900.033.82995633
TCGGTTG1950.033.04787434
ATCAACG24250.032.9409563
Produced by FastQC (version 0.11.5)