FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937171
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14040.14981257422604838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATATA150.00227993169.56947
AAGTTTA150.002371479868.8817824
CTAGATT150.00237902168.826664
TATAGGA200.00707196652.272650
ATATAGG200.007090114552.2387349
GTATCAA28200.042.5986331
GGTATCA10750.039.703851
TGGTATC4000.039.5774422
TATCAAC30350.039.4612122
GTGGTAT4300.036.8221171
GTATAAG853.0358933E-936.445421
TATAAGA905.315087E-934.415162
AACGCAG35700.033.3529746
CAGAGTA35150.032.8014110
ATAAGAG1056.6029315E-1032.77463
AGAGTAC35350.032.51846711
GCAGAGT36950.032.1349539
CAACGCA37450.031.702535
TCCTAGA553.3039274E-431.286512
ATCAACG38350.031.1380043
Produced by FastQC (version 0.11.5)