FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337494
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58250.040.9922331
GGTATCA23550.040.4988021
GTGGTAT7900.038.650151
TGGTATC8300.037.202232
TATCAAC64550.036.8865242
AACGCAG74200.032.135536
ATCAACG75000.031.9299813
CAACGCA76250.031.3615465
AGAGTAC75800.030.85956611
TCAACGC77450.030.8313354
CAGAGTA75800.030.81431810
GCAGAGT78350.030.3805129
ACGCAGA79950.029.8639057
CGCAGAG81000.029.3909768
TCCATAG3401.6370905E-1124.48792570
AGTACGG81700.024.14086713
GAGTACG83300.024.00659812
GTGTATA1902.910383E-1123.4735091
CTAAACT953.1833976E-421.6686654
TCGTATG1605.4729753E-821.65266640
Produced by FastQC (version 0.11.5)