FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337494
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21160.15820631718721728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9100.042.5747071
GGTATCA26750.040.6303061
GTATCAA67100.040.5707131
TGGTATC10050.038.8797572
TATCAAC75900.035.8561332
AACGCAG83300.032.6321266
ATCAACG87250.031.1513043
CAACGCA87300.031.1346245
CAGAGTA85800.031.00213610
AGAGTAC86050.030.951911
TCAACGC88500.030.633864
ACGCAGA89150.030.4523667
GCAGAGT88750.030.3964989
CGCAGAG89850.030.1006648
GAGTACT18950.024.96244212
AGTACTT18900.024.84711313
ATACAGT1001.6440146E-523.9946926
GTACTTT19750.023.77685514
TACTTTT20050.023.76300415
AGTACGG91900.023.7224113
Produced by FastQC (version 0.11.5)