FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992451
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25400.2559320309012737No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22530.2270137266222715No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17540.17673416622080082No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16390.16514669238078253No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15890.16010866027642673No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15890.16010866027642673No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15060.15174552698319616No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC15020.15134248441484768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14550.14660673423675324No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14390.14499456396335939No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14260.1436846756162269No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13930.14035957442735209No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGTTCAGATTCAGGGAGAACTGGTTCTTGGACTTGTCCACCGA13830.13935196800648092No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13280.13381013269168957No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGACTTTACACTGAA13210.13310480819707976No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13130.13229872306038282No Hit
GGCACAGACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGGTCTA12960.13058579214490185No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12790.12887286122942088No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGGTCTACAAAAT12500.12595080260889455No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAG12290.12383482912506512No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT12090.1218196162833228No Hit
GTGTAAAGTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG11990.12081200986245166No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11420.11506865326348606No Hit
GTCTCTGGTGGCTCCATCAGAAGTAGTAATTGGTGGGCTTGGGTCCGCCA11220.11305344042174374No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA11080.11164279143252413No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11070.11154203079043702No Hit
GTCTACAAAATCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA10310.10388422199181621No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10220.10297737621303218No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC10180.10257433364468371No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10140.10217129107633524No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9940.10015607823459294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.040.2709851
GTATCAA17750.035.7623521
GTGGTAT2950.034.8940931
TATCAAC19600.032.37542
ATCAACG20950.030.2876323
TCAACGC21100.030.0723174
CAACGCA21850.029.0415485
AACGCAG22300.028.459816
TGGTATC3650.028.1921272
CGTCTTT950.008456442527.09737270
CGCAGAG23700.026.6352338
AGAGTAC24100.026.04948611
ACGCAGA24900.025.7649487
CAGAGTA24500.025.6254810
TGTTAGA5800.024.41099570
AGTACGG22700.024.33006313
GAGTACG23550.023.45309312
GCGTAGA12600.022.47361470
GTATAGC953.1802116E-421.6710681
TACGGGA8100.021.17416815
Produced by FastQC (version 0.11.5)