FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899542
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67560.3556646812758023No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACGCGGGAATCAGGGGTCCCT63330.3333961554943244No Hit
GCTTAGGAGGCTGTCCTGATTTCTGCTGGTACCAAGCTAAGTACTTCTTATTGTTGGCGGCGTATAAAATACT59620.3138651317001677No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53690.2826470801909092No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC51820.2728026018903504No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47460.24984970061204229No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT46390.24421676383043914No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC45650.24032108792540519No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44750.2355831037165801No Hit
ATATTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT44750.2355831037165801No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC44440.2339511313779848No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43640.22973958985902918No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42460.2235275661185696No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT42100.2216323724350396No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40260.21194582694144168No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38260.20141697314405263No Hit
ACCTACGACAACCCGTCCCTCAGGAGTCGGGTCACCATATCCGTAGACAC36130.19020374384983327No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG35820.18857177151123797No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35800.18846648297326407No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34770.18304412326760872No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33340.17551599280247554No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32860.1729890678911022No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG32380.17046214297972878No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG32200.16951454613796377No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATA32180.1694092575999899No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30560.16088088602410477No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC30280.15940684649247028No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30140.15866982672665306No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA29980.15782751842286194No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29940.15761694134691417No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29410.15482679509060607No Hit
ATTCAGTGCCAGCGGGTATGGGACAGATTTCACTCTCACCATCAGCAGCC29100.15319482275201077No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC28340.14919385830900292No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT28120.1480356843912901No Hit
ATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA27850.14661428912864258No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC27380.14414000848625616No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC27040.14235010334070003No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCATACCCGCTGGCA26960.14192894918880447No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT26850.14134986222994805No Hit
GTCCCATACCCGCTGGCACTGAATCGGTCAGGGACCCCTGATTCCCGCGT26690.14050755392615694No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26500.139507312815405No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25940.13655923375213602No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25790.13576956971733187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24640.12971547878383316No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24210.1274517752173945No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA24070.12671475545157726No Hit
GTATGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG23990.1262936012996817No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23770.12513542738196892No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG23470.12355609931236056No Hit
GACCGATTCAGTGCCAGCGGGTATGGGACAGATTTCACTCTCACCATCAG23340.12287172381553026No Hit
GTACTTAGCTTGGTACCAGCAGAAATCAGGACAGCCTCCTAAGCTGCTCA23190.1220820597807261No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23010.12113446293896107No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA22710.11955513486935272No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22660.119291913524418No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA22010.11587003604026655No Hit
GTCTCTGGTGGCTCCATCAGAAGTAGTAGTCATTACTGGGCCTGGATCCG21950.11555417042634487No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCATACCCGCTGGCACTGAATCGGTCAGGGACC21690.11418541943268429No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21680.11413277516369735No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG21230.1117637830592848No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT21190.11155320598333703No Hit
GTGTAGCAGCTCGGTTTGATGCTTTTGAAATCTGGGGCCAAGGGACAACG20870.10986858937575479No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA20690.10892099253398976No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20570.10828926130614643No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA20470.10776281861627698No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC20440.10760488580931614No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20420.10749959727134224No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20260.10665728896755113No Hit
CAATAAGAAGTACTTAGCTTGGTACCAGCAGAAATCAGGACAGCCTCCTA20250.10660464469856418No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTATGGGACAGATTTCACTCTCAC20220.10644671189160335No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19910.10481473955300805No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCATTCACTTT19820.10434094113212554No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACGCCGCCAACAATAAGAAGTA19800.10423565259415164No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC19770.1040777197871908No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACGCCGCCAA19710.10376185417326914No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT19590.1031301229454258No Hit
GCTCATTTACTGGGCATCTACGCGGGAATCAGGGGTCCCTGACCGATTCAGTGCCAGCGGGTATGGGACAGATTT19540.10286690160049106No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG19520.10276161306251717No Hit
ATTTATTATAGTGGGGCCACCTACGACAACCCGTCCCTCAGGAGTCGGGT19480.1025510359865694No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19120.10065584230303935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4650.041.449941
TCGTATG1850.041.1617640
GGTATCA11900.039.624381
GTATCAA26750.037.570581
TGGTATC4950.036.847022
CGTATGC2000.036.36514741
CTTATAC2150.035.2186781
ATACACA2850.033.809974
CTCGTAT1950.033.70435739
GACTAGG2250.033.67113526
TATCAAC30700.032.395982
CGAGACT2400.031.55089823
TATACAC2400.031.5459163
ATCAACG31500.031.4639763
TCAACGC31650.031.4235924
CAACGCA31750.031.324625
AACGCAG31700.031.1569066
TCGTTAT450.00460435930.69260435
CTCGTTA450.004612099430.68206634
GTATGCC2400.030.3211642
Produced by FastQC (version 0.11.5)