FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899542
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66040.3476627523897866No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACGCGGGAATCAGGGGTCCCT64850.3413980843803401No Hit
GCTTAGGAGGCTGTCCTGATTTCTGCTGGTACCAAGCTAAGTACTTCTTATTGTTGGCGGCGTATAAAATACT53410.2811730406592747No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50810.26748553072266895No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT49460.26037855440943136No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC47690.251060518798742No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT46910.24695426581776025No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44230.23284560172925897No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43430.22863406021030333No Hit
ATATTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT43260.22773910763752525No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42900.22584391395399522No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC42300.2226852578147785No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC42290.22263261354579156No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40740.21447275185281506No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36570.19252009168525885No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35480.18678186636568184No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34290.18051719835623534No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33350.17556863707146247No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATA32460.17088329713162437No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG32110.16904074771708127No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32080.16888281491012044No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31130.16388160935636065No Hit
ACCTACGACAACCCGTCCCTCAGGAGTCGGGTCACCATATCCGTAGACAC31130.16388160935636065No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA30970.1630393010525695No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30940.1628813682456087No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30920.16277607970763477No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30390.1599859334513267No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA29790.15682727731211No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC29280.15414241959377575No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29170.15356333263491936No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC28270.1488253484260943No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28170.14829890573622484No Hit
ATTCAGTGCCAGCGGGTATGGGACAGATTTCACTCTCACCATCAGCAGCC27340.1439294314103084No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC27030.1422974590717131No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT26790.1410339966160264No Hit
ATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA26680.14045490965717No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT26450.13924409147047026No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26430.13913880293249636No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC26100.13740154205592717No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT25700.13529577129644935No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCATACCCGCTGGCA25700.13529577129644935No Hit
GTCCCATACCCGCTGGCACTGAATCGGTCAGGGACCCCTGATTCCCGCGT25480.13413759737873654No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT25420.1338217317648149No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT25050.13187389381229792No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24900.13108422977749373No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24860.13087365270154594No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24520.1290837475559898No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT23970.12618831276170783No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23880.1257145143408253No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23740.12497749457500808No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23590.12418783054020391No Hit
ATTTTATACGCCGCCAACAATAAGAAGTACTTAGCTTGGTACCAGCAGAA23540.12392460919526918No Hit
GTATGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG23260.1224505696636347No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23200.12213470404971304No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA22980.12097653013200024No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22160.11665970007507073No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA22130.11650176726810989No Hit
GTACTTAGCTTGGTACCAGCAGAAATCAGGACAGCCTCCTAAGCTGCTCA22060.11613325738520128No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21940.11550152615735793No Hit
GACCGATTCAGTGCCAGCGGGTATGGGACAGATTTCACTCTCACCATCAG21940.11550152615735793No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA21820.11486979492951459No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT21770.11460657358457987No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21000.11055296487258506No Hit
GTGTAGCAGCTCGGTTTGATGCTTTTGAAATCTGGGGCCAAGGGACAACG20810.10955272376183313No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20740.1091842138789245No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA20730.10913156960993754No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG20700.10897363680297673No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACGCCGCCAA20490.10786810715425087No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20110.10586762493274694No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTATGGGACAGATTTCACTCTCAC20060.10560440358781222No Hit
GCTCATTTACTGGGCATCTACGCGGGAATCAGGGGTCCCTGACCGATTCAGTGCCAGCGGGTATGGGACAGATTT20020.10539382651186444No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG19750.10397243124921692No Hit
GTCTCTGGTGGCTCCATCAGAAGTAGTAGTCATTACTGGGCCTGGATCCG19700.1037092099042822No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCATACCCGCTGGCACTGAATCGGTCAGGGACC19620.10328805575238661No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19420.10223517037264772No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19400.10212988183467384No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACGCCGCCAACAATAAGAAGTA19400.10212988183467384No Hit
ATTTATTATAGTGGGGCCACCTACGACAACCCGTCCCTCAGGAGTCGGGT19300.10160343914480438No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19160.10086641937898713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5750.044.8395232
GTGGTAT6000.043.5544781
GTATCAA30150.037.4073561
GTAGTTT2500.036.06057470
GGTATCA13350.035.029031
TATCAAC34500.032.483742
ATCAACG35700.031.2930873
TCAACGC36250.030.723474
ACGTCTA450.004692841330.57340221
AACGCAG36900.030.1830646
CAACGCA37050.030.1536465
CGTATAA16750.028.53324561
GCGTATA16750.028.28994460
CGCAGAG39950.027.877998
AGAGTAC39550.027.63846611
GCAGAGT41000.026.9963659
GTATAAA18050.026.31681662
TATAAAA19050.025.34506263
AAATACT19100.025.03385467
GGCGTAT18950.024.97608659
Produced by FastQC (version 0.11.5)