FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011257
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16520.16336104471959156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG150.002382839868.7992216
ATAAACG200.007449875651.589213
GTGGTAT4450.047.1615141
TGGTATC4950.042.391442
GTATCAA36550.041.3233071
GGTATCA14200.039.4927371
GTATAAG1050.039.319671
TAAGGGA350.001368515939.3060654
GTGTACA901.2732926E-1038.227461
ATAGCGA451.02000035E-438.214233
TATCAAC39750.037.9044722
TATAAGA1100.037.5268442
TAAGAGA1100.037.519424
ATAAGAG1250.035.7685173
CAGAGTA43850.034.04995710
AGAGTAC44100.033.85693411
GCAGAGT45550.033.0057459
AACGCAG46150.032.873196
TGAAATT800.003302040332.84514670
ATCAACG46650.032.2179153
Produced by FastQC (version 0.11.5)