FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341172
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38880.2898957031611158No Hit
GTGTTAGCACCTACGTAGCCTGGTACCAACAGACACCTGGCCAGGCTCCC31220.23278147769264496No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26940.20086909061626695No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA26130.19482959680041037No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATC24530.18289973247279245No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23830.1776804168294596No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23690.17663655370079304No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTGTT22840.170298813276746No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTGCTACGCTG22600.1685093336276033No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20850.1554610445192712No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT20330.1515838386127954No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20000.14912330409522417No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19870.14815400261860523No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18670.13920660437289176No Hit
GCCTAGAGCCTGAAGATCTTGCAGTTTATTATTGTCAGCAGCGTAGCAAC18190.1356276450746064No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC18120.1351057135102731No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCACAGTGTTAGCAC18030.1344346586418446No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17430.12996095951898787No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16500.12302672587855995No Hit
GCGTAGCAACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT16020.11944776658027456No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15660.11676354710656053No Hit
CTCCTGCAGGGCCACTCACAGTGTTAGCACCTACGTAGCCTGGTACCAACAGACACCTGGCCAGGCTCCCAGGCT14930.11132054650708485No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14900.111096861550942No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATCTTGCAGTTTATTATTGTCAGCAGCGTAGCAACTGG14740.10990387511818021No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13900.10364069634618081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)