FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699578
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAGC150.002365016468.927372
CTCACTA200.00683058252.73222763
GCAGTTA200.00684832952.6976462
TATACAC352.286156E-549.2338333
TGGTATC2400.047.3875662
GGTATCA7800.046.4033741
TTATACA451.873892E-645.9515762
GTGGTAT2550.044.6096271
CTTATAC405.0248116E-543.088851
GTATCAA21200.042.6010481
TATCAAC23200.038.7716452
CAGAGTC1600.034.45629510
CAACGCA27750.032.414495
ATAAGAG1305.456968E-1231.812633
GCAGAGT28450.031.4890569
AGAGTAC26750.031.30048411
TGACTCA450.00422878931.2282362
AACGCAG28900.031.1246436
CAGAGTA26950.031.06819710
ATCAACG29300.030.9349783
Produced by FastQC (version 0.11.5)