FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699578
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14320.2046948303119881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAA200.007058142752.2970650
GACTAGC200.00711080852.1988447
ATGGTAA200.007380623451.70953823
TCATATG200.007405714651.66517318
CAGGTTA200.007409902351.65778416
GTGTATA405.042257E-543.0635451
TGTATAA405.0529066E-543.0481532
GTATCAA20700.041.440861
ATAAGAG1000.041.320323
GTATAAG955.456968E-1239.890441
TATAAGA955.456968E-1239.8761862
AATACTG350.001359799539.35555
GTGGTAT3250.038.160931
TATCAAC22700.037.6244662
TGGTATC3500.035.4224852
GGTATCA8000.035.312111
CAGAGTA25650.032.75789310
AGAGTAC25800.032.56743611
AACGCAG27150.031.7090826
GCAGAGT27300.031.6609959
Produced by FastQC (version 0.11.5)