FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922018
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT89250.4643556928186937No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTT70080.3646167725796533No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC60120.31279623812055873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59800.3111313213507886No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC59300.3085298888980228No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG57270.2979680731397937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT56760.2953146120379726No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC55270.28756234332873054No Hit
CTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG53110.2763241551327823No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52430.27278620699702083No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGACT49360.25681341173703887No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG49070.25530458091443475No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA48220.2508821457447329No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT46120.2399561294431166No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT44830.2332444337149808No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC43280.22517999311140688No Hit
GTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT42170.21940481306626683No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40860.21258906004002046No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT40400.21019574218347592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38430.19994609831957866No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38040.19791698100642138No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA37740.1963561215347619No Hit
CCCCTGGAGATCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTG37660.19593989234231937No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCTCCATCTCCT37210.19359860313483016No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGT36820.19156948582167285No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36250.18860385282551986No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG35640.18543010523314557No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33960.17668929219185253No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33830.1760129197541334No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCC33440.17398380244097608No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTACAGGAGATGGAGGCC33270.17309931540703571No Hit
CTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTC33210.17278714351270383No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA32840.17086208349765714No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32800.1706539689014359No Hit
CTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA31900.16597139048645748No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31270.16269358559597258No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30680.15962389530170892No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC30230.15728260609421973No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30150.15686637690177718No Hit
GAGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT29810.15509740283389645No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC29720.1546291449923986No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29370.15280814227546255No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT29240.15213176983774346No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC29180.15181959794341157No Hit
CTCCCTGCCCGTCACCCCTGGAGATCCGGCCTCCATCTCCTGTAGGTCTA29060.15119525415474777No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28860.15015468117364145No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28790.14979048063025424No Hit
GCTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT28640.1490100508944245No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG27530.14323487084928443No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA27140.14120575353612713No Hit
GTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTTCACTGGCAGTGGATC27080.14089358164179525No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26550.1381360632418635No Hit
GTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGAC26280.13673128971736997No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT26160.13610694592870617No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26030.13543057349098706No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA25800.1342339145627148No Hit
CTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCT25610.13324537023066382No Hit
CTCCTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT25590.13314131293255319No Hit
CCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTG24790.12897902100812791No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24420.12705396099308122No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23860.12414035664598355No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23820.12393224204976228No Hit
GAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGACTCCTCGGACTTTCGGCGGAGGGA23780.12372412745354103No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGA23740.12351601285731975No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23400.12174703878943902No Hit
GCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGC23070.12003009337061359No Hit
GTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAG23050.11992603607250296No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23050.11992603607250296No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22890.1190935776876179No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22470.11690837442729464No Hit
GTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAAAATCAGCAGAG22260.11581577279713301No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22200.11550360090280111No Hit
AGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT21930.1140988273783076No Hit
CACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGC21750.1131623116953119No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTACTATGC21530.11201768141609497No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT21510.11191362411798432No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCA21510.11191362411798432No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21510.11191362411798432No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21270.11066493654065676No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21140.10998856410293764No Hit
GGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTAT20780.10811553273694628No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20750.10795944678978033No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT20650.10743916029922716No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC20220.10520192838984858No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT20180.10499381379362731No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20130.10473367054835074No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTT19910.10358904026913379No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAGTCTGTGCCGGATCCACTGCC19810.10306875377858063No Hit
ACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGC19500.10145586565786585No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG19380.10083152186920205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4900.046.1266021
TGGTATC5300.042.6342542
GGTATCA13300.037.5928081
GTATCAA31450.037.457721
TATCAAC37050.031.7878252
AACGCAG38500.031.1257956
ATCAACG38300.030.9283393
TCAACGC38850.030.7556554
GTAGTTT2151.4042598E-930.54708970
CAACGCA39250.030.4430125
AGAGTAC40850.028.6639411
CAGAGTA41050.028.52503210
CGCAGAG41450.028.414968
TGTTAGA15750.027.93846370
ACGTTAT500.00797100127.43138126
ACGCAGA44450.027.0363797
CCCTAGT801.0067693E-425.68078815
CCGTTAA951.1142745E-525.23090416
GTATAAG3150.025.0046541
GAGTACG39800.024.43013412
Produced by FastQC (version 0.11.5)