FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298418
Sequences flagged as poor quality0
Sequence length20-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA350.00584103654.8605570
GTATCAA42150.042.2000541
GGTATCA15700.038.3493921
TATCAAC50150.035.7371522
AACGCAG56700.031.9121326
ATCAACG57800.031.4238363
CAACGCA58250.031.1810765
AGAGTAC56150.031.1748311
CAGAGTA56800.030.87862210
TCAACGC60600.030.1989734
GCAGAGT59550.030.145669
ACGCAGA61250.029.58977
TGGTATC6100.029.3242322
CGCAGAG61750.029.2387228
GTGGTAT6350.028.7158851
GTAATAC500.00784608927.5196653
TATACAC1800.026.7552283
ATGCTAT1208.4104773E-426.66832470
CTTATAC1901.8189894E-1225.3509651
ATAAGAG2450.025.273163
Produced by FastQC (version 0.11.5)