FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298418
Sequences flagged as poor quality0
Sequence length20-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22250.17136238098978912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49400.041.123221
GGTATCA19450.037.819931
TATCAAC55650.036.3096162
AACGCAG60350.033.4805686
CAGAGTA59900.032.58725410
AGAGTAC60300.032.37108211
CAACGCA62750.032.253565
GCAGAGT62850.032.041929
ATCAACG63850.031.6978973
ACGCAGA64200.031.47527
TCAACGC64400.031.4271834
CGCAGAG64600.031.227118
GTGGTAT7800.027.7633911
TGGTATC8000.027.062012
AGTACGG68800.025.42468513
GAGTACG69600.025.37932612
AGAGTCG2450.025.2483311
GTAGTTT2051.1551292E-625.08229870
GCTTTAC700.001362745724.552661
GTGTATA1452.0954758E-823.7060171
Produced by FastQC (version 0.11.5)