FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960892
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTGCTTAGCTTGGTACCAGCTGAA79630.40609069749889337No Hit
ATATTATAGTGCTCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT68460.34912682595471856No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68060.3470869379853659No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA57890.2952227863645729No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT57320.29231594600824523No Hit
GTCTATTACTGTGCGAGACATACCCCCTCCGTCGGCACGCGACGATTTTTGGAGTGGTTATCACCGGGTTTTGA56440.28782819247566926No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC56020.2856863101078489No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48730.2485093518663955No Hit
GCTTAGGAGGCTGTCCTGGTTTCAGCTGGTACCAAGCTAAGCAGTTCTTATTGTTGGAGCTGTATAAAACACT42840.21847200151767662No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42630.21740106033376647No Hit
TCCGTAGACAGGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTCTATTA42110.21474920597360794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39940.2036828137398694No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38490.19628821985096578No Hit
ATCTACTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT37160.18950559235286799No Hit
GTCTCTGGTGGTTCCATCAGGAGTAATTACTACTGGGGCTGGATCCGCCA36870.18802667357508726No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36560.1864457603988389No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36410.18568080241033164No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35000.1784901973183633No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32900.16778078547926148No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32550.16599588350607786No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32520.1658428919083764No Hit
GTCCCTGACCGACTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC32520.1658428919083764No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGACTGGAGA32230.16436397313059567No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31860.1624770767589444No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC30570.15589843805778186No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC30550.15579644365931422No Hit
CACCTGCACTGTCTCTGGTGGTTCCATCAGGAGTAATTACTACTGGGGCT29930.15263461730681752No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29760.15176766491984262No Hit
GTCTACGGATATGCTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGCTCCCACTATAGTAGATAGTCCCAAT27640.14095625868227318No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC27520.14034429229146736No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27150.1384573959198161No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26830.1368254855443339No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25150.1282579560730525No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24740.12616707090446594No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24610.1255041073144263No Hit
GTTTATTACTGTCAGCAATATTATAGTGCTCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA23250.11856848821862705No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23120.11790552462858739No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22720.11586563665923469No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22580.11515167586996122No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22190.11316278509984233No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21910.11173486352129541No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20690.10551320521476959No Hit
GTCAGGGACCCCGGATTCCCGAGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCAGCTGGTA20540.10474824722626233No Hit
GCTTGGTACCAGCTGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACTCGGGAATCCGGGGTC20450.10428927243315797No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA20280.10342232004618307No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACTCGGGAA20220.10311633685078016No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20080.1024023760615067No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20060.10230038166303906No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT20030.1021473900653376No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGTCTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG19970.1018414068699347No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19870.10133143487759654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG200.007437391751.61291511
TACGGAT17250.044.925670
GGTATCA16150.040.7051931
GTGGTAT5900.037.9184271
TCGTATG1200.037.49205440
GTATCAA36100.037.4691931
CGTATGC1600.036.78331841
TGGTATC6100.036.105442
CGCGTAT400.002564344634.58853539
CGACATA750.002726086934.15830670
TATCAAC40300.033.217512
TAATAGA15100.031.4502892
TCAACGC43500.031.0112534
ATCAACG43350.030.9597913
AATAGAC15250.030.9152833
CAACGCA44050.030.7021755
GTAATAG15700.030.6914461
AACGCAG43750.030.676736
CTACGGA18100.030.44658969
TAACGGC2000.029.35285436
Produced by FastQC (version 0.11.5)