FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960892
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTGCTTAGCTTGGTACCAGCTGAA72890.3717185852153No Hit
ATATTATAGTGCTCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT70020.3570823890351942No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67940.34647497159456003No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC64630.3295948986481662No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA55490.2829834585484565No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT53830.27451792347564274No Hit
GTCTATTACTGTGCGAGACATACCCCCTCCGTCGGCACGCGACGATTTTTGGAGTGGTTATCACCGGGTTTTGA48430.24697943588938095No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42550.21699308273989593No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40640.20725261768623665No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38070.19414633748314544No Hit
GCTTAGGAGGCTGTCCTGGTTTCAGCTGGTACCAAGCTAAGCAGTTCTTATTGTTGGAGCTGTATAAAACACT37130.18935260075516652No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36940.18838365396972398No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36830.18782268477815198No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36270.18496684162105817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36090.18404889203484945No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34020.1734924717934491No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC33800.17237053341030512No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC33750.172115547414136No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31580.16104915518039747No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT31160.15890727281257713No Hit
GTCCCTGACCGACTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC30070.15334857809609095No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC29710.1515126789236735No Hit
TCCGTAGACAGGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTCTATTA29400.14993176574742514No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG28060.14309814105009352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27820.1418742082684819No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27690.14121124467844226No Hit
GTCTCTGGTGGTTCCATCAGGAGTAATTACTACTGGGGCTGGATCCGCCA27250.13896736791215425No Hit
GTCTACGGATATGCTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGCTCCCACTATAGTAGATAGTCCCAAT26310.13417363118417536No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26030.13274570960562845No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25650.13080781603474337No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24870.12683003449450556No Hit
GTTTATTACTGTCAGCAATATTATAGTGCTCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA23950.1221382921649943No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23910.12193430336805904No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23890.1218323089695914No Hit
ATCTACTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT23690.12081236498491504No Hit
CACCTGCACTGTCTCTGGTGGTTCCATCAGGAGTAATTACTACTGGGGCT23530.11999640979717394No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA23430.11948643780483575No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGACTGGAGA23020.11739555263624922No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22760.11606962545616996No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22650.11550865626459796No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22560.1150496814714936No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22380.11413173188528486No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA22210.11326477949830996No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22170.11306079070137467No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21630.11030694194274851No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20330.10367730604235216No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA20320.10362630884311833No Hit
GTCAGGGACCCCGGATTCCCGAGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCAGCTGGTA19690.1004134852913878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT16500.042.0923370
GTATCAA36450.038.8636631
GGTATCA15100.037.1154441
GTGGTAT6600.035.9458851
TATCAAC41650.034.0045972
TGGTATC7100.033.407672
ATCAACG44250.032.1611373
CAACGCA44500.032.0601585
TCAACGC44500.031.9032124
AACGCAG44600.031.8349256
AGAGTAC47900.029.42714111
CGCAGAG48600.029.0740328
CAGAGTA48850.028.71410810
GCAGAGT49550.028.6553769
CTATTAC14950.028.281883
TGTGCGA14900.027.91893410
CTACGGA17450.027.8264669
ACGCAGA51300.027.6108387
TACGGGT3700.026.94610615
TACTGTG16350.026.4943057
Produced by FastQC (version 0.11.5)