FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380628
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26130.1892616982996144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAA200.00714010952.14731243
CCTAATG200.007287077651.87930736
ATGTTAA200.00747153251.5523516
GTATCAA55450.041.9079781
GGTATCA20550.037.9721871
TATCAAC61350.037.922752
GTATAAG2200.037.5007671
TATAAGA2200.037.48992
TAAGAGA2350.035.0956574
TTATAGA400.002525735434.69496540
TGTATAA1102.910383E-1134.365742
AACGCAG69250.033.7454226
AGAGTAC68200.032.70288511
CAGAGTA68300.032.65500310
CAACGCA72400.032.372155
ATAAGAG2550.032.3430563
GCAGAGT71700.032.160979
ACGCAGA74000.031.532897
ATCAACG75250.031.0992983
TCAACGC75650.030.9802884
Produced by FastQC (version 0.11.5)