FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105228
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACACTACCCCTGGCACCTTCGGCCAAGGGACCAACGTGGAAATCATACGAACTGTGGCTGCACCAT22220.20104449036759836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20960.18964412772749153No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20460.1851201742988777No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17350.1569811839728997No Hit
GTGTAACTCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACT16440.14874758873282254No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGAAGATACA16350.14793327711567206No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15510.14033303535560085No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15290.13834249584701075No Hit
GTCTATTACTGTGCGAGAGGGAATTGTGGTGGTGACTGCTATTCCTATTA14370.1300184215383613No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14170.12820884016691578No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14160.12811836109834351No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13960.12630877972689797No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13610.1231420123268683No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13480.12196578443542871No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12690.11481793801821888No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11980.10839392414958722No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11190.10124607773237738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20200.039.4209751
TATCAAC22900.034.4655232
ATCAACG24150.032.252393
AACGCAG24550.031.8681016
CAACGCA24500.031.6515945
TCAACGC24500.031.6515944
GTGGTAT4000.030.8910941
AGAGTAC25800.030.06082311
CGCAGAG26500.029.3962638
TATATAT605.5947126E-428.5963922
GCAGAGT27450.028.5039259
CAGAGTA28000.027.82146310
ACGCAGA28600.027.3577887
AGTACGG25500.027.05004913
TAACGGC658.6020096E-426.5673636
TTAACGG658.647861E-426.54318435
GAGTACG26450.026.07849712
TGTTAGA3551.8189894E-1225.11377770
GTATGTA5800.024.263121
TATGTAC5650.023.6869242
Produced by FastQC (version 0.11.5)