FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842552
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAAA200.00697769252.4498358
TTTAGGT200.007367751.73338726
ATATAAC200.007367751.73338726
GATATAA200.00737462351.721125
ATGTGTA850.048.6526811
CTTATAC451.8710361E-645.9633941
TATACAC451.8733317E-645.9552083
GTGTATA950.043.5365213
GTATCAA23350.042.6662331
TGTGTAT1000.041.35723512
TGGTATC3750.040.4405862
TGTAAAA350.001257380839.9882659
GTGGTAT3800.039.9155771
CCTTAGA350.001354082439.390182
GAATAAG350.001354477539.387847
GGTATCA9250.039.1309971
TATCAAC26650.037.634992
TGTATAA1100.037.59971614
GTATAAG1950.035.356461
TATAAGA1950.035.3501632
Produced by FastQC (version 0.11.5)