FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842552
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13240.15714163636191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTAA250.001385782578.86336570
GTGTATA552.6411726E-950.0852171
TGTATAA652.1827873E-1047.671612
GGTATCA11300.045.70831
GTGGTAT3150.042.6320571
GTATCAA28200.041.7598841
TGGTATC3550.037.823962
TATCAAC31150.037.8006062
TCTAAGG400.002622113634.4294972
TAAGAGA1056.566552E-1032.7899974
CAGAGTA35300.032.6777710
AGAGTAC35700.032.3116311
GCAGAGT36550.032.1254549
AACGCAG36900.032.100696
CAACGCA37400.031.303265
TATAAGA1101.0804797E-931.2995472
ATCAACG38200.030.6440563
AGGAGTA902.0301923E-730.6042
CGCAGAG38850.030.4008248
TCAACGC39000.030.103744
Produced by FastQC (version 0.11.5)