FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781321
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT94630.5312349655115501No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATTAGGCTGAAGGCTGCTGATGGTGAGAGTGAATTCTGTC92910.5215792100356983No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT83420.46830414057881764No Hit
GGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA71510.4014436477198663No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63220.3549051518507894No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53750.3017423586203722No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50910.2857991344625702No Hit
AGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC49830.2797362182335469No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG49520.2779959367233643No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC49430.27749069370427903No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48970.27490834049562096No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47480.26654376162409804No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA46020.2583475970922703No Hit
CTATAGACCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC44760.25127419482507646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44430.24942163708843043No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40450.227078668022215No Hit
GAATTCACTCTCACCATCAGCAGCCTTCAGCCTAATGATTTTGCAACTTA38510.21618787405526574No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG38110.21394234952599786No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCG37670.21147227254380316No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37260.20917060990130357No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG35980.20198493140764634No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35980.20198493140764634No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35940.20176037895471957No Hit
GTAGTAAACGGCCGTGTCCGCGGCGGTCACAGAGGTGAGTTTCAGGGAGA35300.19816753970789092No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT34730.1949676672536842No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34320.19266600461118463No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32880.18458211630582022No Hit
GTGGAGACTAGACGCCCTATAGACCAGGAGGTTAGGGGCTTTCCCTGGTT32830.18430142573966174No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATTATTGGTTGGCCTGGTATCAGCAGAAA32770.18396459706027157No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA32540.18267342045594254No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT32220.18087700083252822No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31690.17790168083124827No Hit
CCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAAT31360.17604912309460227No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30840.173129941206554No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30230.1697055162994205No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29970.16824592535539634No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29480.16549515780704321No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29020.16291280459838514No Hit
GGCCAAAAGTGTACGAATAACTATTATACTGTTGGCAGTAATAAGTTGCA27610.15499733063271584No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26290.14758709968613182No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26280.1475309615729001No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25710.14433108911869336No Hit
CAGTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25150.14118735477771832No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACC24800.13922252081460892No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24500.13753837741765804No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24490.13748223930442632No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24280.13630333892656069No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC24100.13529285288839013No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23620.1325982234532687No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23020.12922993665936683No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTTG22900.1285562793005865No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCAT22680.12732124080948914No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22460.1260862023183918No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21930.12311088231711186No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21920.12305474420388016No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21220.11912507627766136No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT21210.11906893816442966No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATTAGGCTGAAGGCTGC20630.11581292759699122No Hit
CCCTATAGACCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAATAATTAATATTCTGA20490.11502699401174746No Hit
GTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT20320.11407264608680862No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20270.11379195552065012No Hit
GCCCCTAACCTCCTGGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGAT20160.11317443627510146No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20100.11283760759571128No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19960.1120516740104675No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19880.11160256910461394No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19870.11154643099138223No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19760.11092891174583357No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19670.11042366872674829No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19280.10823428231071211No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19140.10744834872546834No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19060.10699924381961476No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18930.10626944834760271No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCACTGTGGAGACTAGACGCCCTATAGA18920.10621331023437101No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18770.10537123853589556No Hit
CATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTTCAGCCTAATGATTTTG18710.10503440985650536No Hit
GTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT18670.10480985740357858No Hit
GTTTACTACTGTGCGAAAGCCCCCGGACATAATTGGAATTATGTCCCTGA18320.10284502344046918No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18300.1027327472140058No Hit
CCCCTAACCTCCTGGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCA18230.1023397804213839No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAATAATTA18100.10160998494937183No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18070.10144157060967675No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17840.10015039400534771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)