FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237120
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT91530.4091421112859391No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCG68410.3057949506508368No Hit
GTCTGGGACAGAGTTCACTCTCACCATCCGCAGCCTGGAGTCTGAAGATT65920.2946645687312259No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64870.2899710341868116No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG59180.2645365469889859No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC57390.2565351880989844No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT56780.25380846803032475No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT56160.25103704763267054No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC52360.23405092261479044No Hit
GTATCATGACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT51820.23163710484909167No Hit
GTCATGATACTGCTGACAGAAATAAAGTGCAAAATCTTCAGACTCCAGGCTGCGGATGGTGAGAGTGAACT51680.2310113002431698No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG49490.2212219281933915No Hit
GAGTTCACTCTCACCATCCGCAGCCTGGAGTCTGAAGATTTTGCACTTTATTTCTGTCAGCAGTATCATGACTGG49170.21979151766557No Hit
CTCCCAGGCTCCTCATCTATGCTGCCTCCACCAGGGCCACTGGTATCCCA49020.2191210127306537No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTCATGATACTG48890.21853990845372623No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT48440.21652839364897725No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48320.2159919897010442No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45130.2017325847518238No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAA43580.19480403375768846No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42950.19198791303103993No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAACAACTTAGCCTGGTACCAGCAGAAACCTGGCCACTCTCCCAGGCT42660.1906916034902017No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40640.18166213703332856No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37220.16637462451723645No Hit
CATCTATGCTGCCTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA37040.16557001859533685No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36910.16498891431840937No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC36400.1627091975396939No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36350.16248569589472178No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36270.16212809326276642No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAACAACTTAGCCTGGTA36060.16118938635388355No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT35160.15716635674438562No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34070.1522940208839937No Hit
GGCCAAAAGTGTACGGAGGCCAGTCATGATACTGCTGACAGAAATAAAGT33770.15095301101416106No Hit
CTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCA33670.15050600772421685No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA33170.14827099127449578No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32540.14545487054784723No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC32440.14500786725790302No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA32070.14335395508510942No Hit
GCCTGGGAGAGTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGTTACTA31120.1391074238306394No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30920.13821341725075098No Hit
GCATAGATGAGGAGCCTGGGAGAGTGGCCAGGTTTCTGCTGGTACCAGGC30520.13642540409097412No Hit
GGATACCAGTGGCCCTGGTGGAGGCAGCATAGATGAGGAGCCTGGGAGAGTGGCCAGGTTTCTGCTGGTACCAGG29210.1305696609927049No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG28990.12958625375482763No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT28780.1286475468459448No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28610.12788764125303964No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC28580.12775354026605634No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28240.12623372908024602No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27300.12203189815477042No Hit
CTGCTACTCTGGCTCCCAGATAGCGCTGGAGAAATAGTGATGACGCAGTC26440.11818766986125018No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26200.11711486196538407No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25950.11599735374052353No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25570.11429874123873553No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25340.11327063367186382No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA25270.11295773136890289No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25030.11188492347303676No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24910.11134851952510372No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24830.11099091689314833No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24310.10866649978543842No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24230.10830889715348305No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24150.10795129452152769No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG24100.10772779287655558No Hit
CCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTCATGATACTGCTGA22850.1021402517522529No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC22460.10039693892147047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3400.043.81864541
TCGTATG3400.043.79100440
CTCGTAT3250.043.6606839
CTTATAC3800.042.4987751
GTATCAA34750.040.4418141
TTATACA4150.039.7398452
TATACAC4200.039.267633
ACGGACT4000.037.82214727
GGTATCA14750.037.5055921
GACGGAC4300.035.97818826
TATCTCG4300.035.33675436
TCCTATC4200.035.2829433
GTAGTTT3350.034.4001770
CTAGTAC400.002649466534.3591773
TATCAAC41100.034.274812
GTGGTAT5400.033.7244031
ACGAGAC4550.033.23165522
ATCAACG42950.032.7992133
TCAACGC43650.032.2732244
CCTATCT4500.032.1851234
Produced by FastQC (version 0.11.5)