FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237120
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT85990.3843781290230296No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCG66120.2955585753111143No Hit
GTCTGGGACAGAGTTCACTCTCACCATCCGCAGCCTGGAGTCTGAAGATT65840.2943069660992705No Hit
CTCCCAGGCTCCTCATCTATGCTGCCTCCACCAGGGCCACTGGTATCCCA57990.25921720783864965No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT57210.25573058217708483No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56720.25354026605635815No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC55070.24616471177227864No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54480.24352739236160775No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT53690.2399960663710485No Hit
GTATCATGACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT52670.2354366328136175No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50760.226898869975683No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50440.22546845944786154No Hit
GAGTTCACTCTCACCATCCGCAGCCTGGAGTCTGAAGATTTTGCACTTTATTTCTGTCAGCAGTATCATGACTGG48800.21813760549277642No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT48420.2164389929909884No Hit
GTCATGATACTGCTGACAGAAATAAAGTGCAAAATCTTCAGACTCCAGGCTGCGGATGGTGAGAGTGAACT47440.2120583607495351No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTCATGATACTG46920.20973394364182518No Hit
CATCTATGCTGCCTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA46520.20794593048204835No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAACAACTTAGCCTGGTACCAGCAGAAACCTGGCCACTCTCCCAGGCT44360.19829065941925333No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT42610.19046810184522958No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAA42350.18930589329137462No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39720.1775497067658418No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39630.177147403804892No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38620.17263267057645545No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT38480.17200686597053355No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38340.17138106136461165No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38290.17115755971963953No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37170.16615112287226433No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAACAACTTAGCCTGGTA36460.16297739951366041No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36170.1616810899728222No Hit
CTTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCA34140.15260692318695465No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC34050.15220462022600487No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32640.14590187383779143No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC32610.1457677728508082No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA32010.14308575311114288No Hit
GCCTGGGAGAGTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGTTACTA31970.14290695179516522No Hit
GGCCAAAAGTGTACGGAGGCCAGTCATGATACTGCTGACAGAAATAAAGT31590.1412083392933772No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30850.13790051494779001No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA30840.1378558146187956No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT29490.1318212702045487No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29450.13164246888857103No Hit
GCATAGATGAGGAGCCTGGGAGAGTGGCCAGGTTTCTGCTGGTACCAGGC28960.1294521527678444No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28130.1257420254613074No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27610.12341760835359748No Hit
CTGCTACTCTGGCTCCCAGATAGCGCTGGAGAAATAGTGATGACGCAGTC27500.12292590473465884No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27400.12247890144471464No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26990.12064618795594335No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26480.11836647117722786No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC25540.11416464025175227No Hit
GGATACCAGTGGCCCTGGTGGAGGCAGCATAGATGAGGAGCCTGGGAGAGTGGCCAGGTTTCTGCTGGTACCAGG25310.11313653268488057No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25310.11313653268488057No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25270.11295773136890289No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24810.1109015162351595No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA24770.11072271491918181No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24770.11072271491918181No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24310.10866649978543842No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24140.10790659419253326No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23850.10661028465169503No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG23460.10486697182091259No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23080.10316835931912459No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22800.1019167501072808No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA22440.10030753826348161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG200.00750111451.50197
GTGGTAT6850.040.1113551
GTATCAA38950.038.181091
GGTATCA17650.035.999371
TATCAAC43750.033.7452552
ATCAACG46750.032.0175673
TCAACGC46700.031.9783334
CAACGCA47250.031.6075135
AACGCAG47150.031.5296236
AGAGTAC49650.029.52844611
CGCAGAG50650.029.1488238
TATAAGA3800.028.91272
TGTTAGA15200.028.71350970
GCAGAGT52050.028.4967299
CTACGTA500.00792001327.467689
CAGAGTA54650.026.88967910
ACGCAGA55300.026.8840547
TTAGGCG658.9049316E-426.4118215
GCAGTAT36300.025.66164270
TATTCTA3058.913048E-1125.62928470
Produced by FastQC (version 0.11.5)