FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345907
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17750.13188132612431616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30900.038.4563641
GTGGTAT5750.034.6426241
TATCAAC35350.033.8008422
ATCAACG35550.033.3196953
GGTATCA13150.032.6463661
AACGCAG36500.032.552586
CAACGCA36600.032.4612275
TCAACGC36800.032.3757134
AGAGTAC37200.030.74111711
CAGAGTA37550.030.4545810
CGCAGAG39050.030.1641758
ACGCAGA39550.030.0433247
GCAGAGT43250.027.2349369
TGGTATC7350.026.627182
AGTACGG38450.026.07789813
GAGTACG39950.024.9277712
ATAATAC851.4915629E-424.2356053
TATCGAC750.001917265723.15411644
GTACGGG43400.022.23075714
ACGGGTA1907.166818E-1021.686116
Produced by FastQC (version 0.11.5)