FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652369
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT208.6575055E-568.7632751
AGTCTGA200.00731239751.8307235
CTTATAC552.6757334E-950.0096551
TTATACA552.6793714E-950.001992
TATACAC605.7862053E-945.8351633
GGTATCA7850.040.732391
GTATCAA18500.040.1428871
GTGGTAT2250.039.7298971
TCGTATG702.1045707E-839.59990340
TACACAT702.257002E-839.2872775
CTCGTAT753.889363E-836.93422739
ATACACA754.145113E-836.6681254
TATCAAC20450.036.3095132
TGGTATC2800.034.376372
GTATAAG1359.094947E-1230.5614591
TATAAGA1359.094947E-1230.5567742
TAAGAGA1359.094947E-1230.5567744
AACGCAG24900.029.8204656
GTGTATA703.9418537E-529.46771613
ATCAACG25600.029.1393433
Produced by FastQC (version 0.11.5)