FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139695
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40660.19002708329925527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72550.039.774981
TATCAAC82900.034.884442
GGTATCA31350.034.872931
AACGCAG87050.033.1827856
CAACGCA87600.032.8555645
TCAACGC87950.032.840924
ATCAACG88100.032.8238953
AGAGTAC92000.030.76784111
ACGCAGA93600.030.754477
CAGAGTA92150.030.71775610
CGCAGAG94050.030.425138
GCAGAGT97650.029.233289
GTGGTAT13550.027.3161621
AGTACGG79150.025.54380413
GAGTACG80450.025.42979612
TGGTATC14300.025.159222
GTACGGG87000.022.76420414
TACGGGA31000.022.21663515
AGTACTT35500.022.10505513
TTAACGG5000.022.08304235
Produced by FastQC (version 0.11.5)