FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638048
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40340.2462687296098771No Hit
CTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT37140.22673328254117095No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36720.22416925511340324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27020.1649524311863877No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25940.15835921780069936No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25570.15610043173338023No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25030.15280382504053605No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24930.152193342319639No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24880.15188810095919045No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24490.14950721834769187No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24470.14938512180351246No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23760.1450506944851433No Hit
GGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT23720.14480650139678447No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG23680.14456230830842562No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTG22800.13919006036453144No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG22320.13625974330422552No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT21530.13143692980913868No Hit
CTCTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20470.12496581296762975No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20150.12301226826075914No Hit
ACCTACTACAACGCGTCCCTCAAGAGTCGAGTCACCATATCTGTAGACACGTCCAAGAACCAGTTCTCCCTGCAA20070.1225238820840415No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA20050.12240178553986208No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19620.1197767098400047No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19280.11770106858895465No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTT18510.11300035163804723No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18260.11147414483580456No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAA17940.10952060012893396No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17620.10756705542206332No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17570.10726181406161481No Hit
GGCCAAAAGTGTACAAGGGGGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTG16760.10231690402234855No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT16680.1018285178456309No Hit
GTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT16570.10115698685264413No Hit
GTAGTAGGTGGTCCCATCATAATAGATTCTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC16430.10030231104338823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)