FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930946
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCACTCACAGAGGTCAGCTTCAGGGAAA31010.3331020274000855No Hit
GTGTATTACTGTGCGGGGTCTGTCGCCCGAGACTTGGAGTGGTTATTTGG29030.3118333394203316No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22960.24663084647229805No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21990.2362113377145398No Hit
GTCTCTGGTGGCTCCGTCACCAGTAATAGTTACTACTGGGGCTGGATCCG21160.22729567558161268No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19300.20731599899457112No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18520.19893742494194078No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17900.19227753274626028No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17310.18593989339875783No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14920.16026708316057No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14200.15253301480429585No Hit
GTATTACTGTGCGGGGTCTGTCGCCCGAGACTTGGAGTGGTTATTTGGGCCCCATAGCTGGTTCGACCCCTGGGG13520.14522861691225913No Hit
CAGTAATACACAGCCGTGTCTGCGGCACTCACAGAGGTCAGCTTCAGGGA13230.1421135060465376No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13070.1403948241895878No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13050.14017998895746905No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12790.13738713093992563No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12660.1359907019311539No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11930.12814921595882037No Hit
CACCATATCCGTGCACACGTCCGAGAACCAGTTTTCCCTGAAGCTGACCT11800.12675278695004866No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11440.12288575277191159No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGCACACGTCCGAGAACCAGTTTTCCCTGAAGCTGA11110.1193409714419526No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG10750.11547393726381552No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10500.11278849686233144No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10430.11203657354991589No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA10390.11160690308567844No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10270.11031789169296609No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC10100.1084917922199569No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10060.10806212175571944No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9960.10698794559512582No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9890.10623602228271027No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT9760.10483959327393855No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9460.10161706479215765No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA9360.10054288863156403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)