FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884890
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA30800.34806586129349415No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25090.2835380668783691No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23750.2683949417441716No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGT20800.23505746476963238No Hit
GTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCAAGACTATGC19940.22533874266858028No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19530.22070539841110193No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19010.21482896179186114No Hit
AGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGGCTCTTATCGGGCC17920.2025110465707602No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16870.19064516493575473No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16400.18533377029913323No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15190.17165975431974595No Hit
ATCCAAGACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC15000.1695125947857926No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14980.16928657799274485No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14390.162619082597837No Hit
GGCACAGACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTA14280.16137599023607455No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTC13990.15809874673688257No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13950.1576467131507871No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAG13630.15403044446202352No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13570.15335239408288034No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12690.14340765518878051No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12500.14126049565482715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12330.1393393529139215No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12210.13798325215563517No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG12070.1364011346043011No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11980.13538405903558634No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11800.13334990789815684No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG11750.1327848659155375No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11600.13108973996767959No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAA11510.13007266439896484No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11490.1298466476059171No Hit
GTGGAGACTGTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCA11340.1281515216580592No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAGCTGTGGAGACTGTCCTGGCTTCTGCAGGTA11290.12758647967543987No Hit
GTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGGCT11160.12611737052062968No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTC11030.12464826136581947No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGTTCTCCAGGGGTGACGGGCAGGGAGACTGGAGA10990.12419622777972404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10780.12182305145272294No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10700.12091898428053204No Hit
CCCCTGGAGAACCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG10100.11413848048910033No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9990.11289538812733785No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA9770.1104092034038129No Hit
CTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAAT9630.10882708585247886No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9610.10860106905943112No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAAAATCAGCAGAG9480.10713195990462092No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9390.10611488433590617No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9250.1045327667845721No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9230.10430674999152437No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC9100.10283764083671416No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9090.1027246324401903No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9080.10261162404366644No Hit
GTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAACCGGCCTCCATCTCCT8850.10001243092361761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3150.039.1236081
TGGTATC3150.036.9417232
GTATCAA15000.036.7435871
TGTTAGA6350.036.40397670
TATCAAC16550.033.0880172
GGTATCA7250.032.108341
TCAACGC17950.029.9336324
ATCAACG18100.029.6855643
AACGCAG18400.029.5735536
CAACGCA18350.029.4676345
GTGTATA704.0594692E-529.3427051
GACCCAT2302.7102942E-928.71618370
AGAGTAC19550.028.36070311
CAGAGTA19850.028.10449810
TATGTAC3850.026.6691592
ACGCAGA20800.026.3272467
CGCAGAG20850.026.099968
GTATACA801.00774545E-425.6748681
CTGTCTT18800.025.2946870
AGTACGG20850.024.78675513
Produced by FastQC (version 0.11.5)