FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778496
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT8490.10905643702729365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA150.002390296468.743532
TATACAC1150.053.8027423
GAGACTA1050.052.3894824
TTGAAAC200.00706958452.276247
CTTATAC1150.050.8234941
TTATACA1150.050.8104362
AGACTAG1100.050.01457225
GACTAGC1150.047.84924326
TACACAT1250.046.74865
ATACACA1450.042.671144
GGTATCA9450.042.5664521
ACTAGCG1300.042.33362227
GTATCAA22850.040.474311
GTGGTAT2750.038.7563131
TCGTATG1200.037.5569140
TATCAAC26100.035.5572
AGGGGGG1601.129938E-732.70990470
TGGTATC3050.032.6813472
CTAGCGC1700.032.38109228
ATGTGTA553.3279482E-431.24705911
Produced by FastQC (version 0.11.5)