FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778496
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13330.1712275978296613No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT7820.10045009865175929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATAAT100.009342764132.210670
ATGTAAT200.007237512651.96677440
GTGTAAC306.419762E-445.8395771
TACTCAT306.4217945E-445.836637
GTATCAA25450.041.0664941
CTAACGC556.8110166E-638.08741852
GTGGTAT4000.037.817651
GGTATCA10200.037.4131851
TGGTATC4150.037.2743872
GTATAAG754.143294E-836.6716581
TATAAGA754.1480234E-836.6669462
TATCAAC28500.036.4257162
AGGCTAA400.002466556434.86115649
GTGTATA400.002640674434.379681
AATGAGA400.002642338434.3752632
CAGAGTA30650.032.86329710
AGAGTAC30850.032.6502411
ATAAGAG851.2465898E-732.353193
TAAGAGA851.2465898E-732.353194
AACGCAG32200.032.026646
Produced by FastQC (version 0.11.5)