FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881223
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1050.058.9988481
TATACAC1200.051.6181343
TAATCTC850.048.80891835
CTAATCT850.048.77554334
ATACACA1350.045.8827864
TGGTATC3100.045.512762
TTATACA1400.044.244112
TGTGTAT551.5016849E-743.78726212
CGCTAAT1350.043.4694332
GACACTC1350.043.348526
CCTAGAC405.076931E-543.015113
GTGGTAT3400.042.5138741
TCGCTAA1400.041.8859531
GGTATCA8750.040.5125471
TGTATAA602.9723378E-740.14060614
GTGTATA602.9723378E-740.14060613
GTATCAA20800.040.0416871
GCTAATC1150.039.0424333
TACACAT1600.038.71365
AGACACT1700.036.4466725
Produced by FastQC (version 0.11.5)